Document Type : Original articles
Authors
1
Environment, Natural Resources and Desertification Research Institute, National Centre for Research P. O. Box 6096 Khartoum Sudan
2
Department of Agronomy, Faculty of Agriculture, University of Khartoum, Khartoum, Sudan
3
Department of Botany, Faculty of Science, University of Khartoum, Khartoum, Sudan
Abstract
The aim of the present study was to estimate the effect of chromium on catechol oxidase and peroxidase activities in ten sorghum genotypes grown in soil amended by different concentrations of chromium. Ten sorghum genotypes, (Tabat, Wad Ahmed, L4, L7, L12, L14, L16, L25, L32 and L34) were obtained from the Department of Agronomy, Faculty of Agriculture University of Khartoum., Seeds were sown in polyethylene bags filled with 2 kg soil (clay and sand, 2:1). Two weeks after sowing, the seedlings were irrigated with eight levels of chromium (Cr-VI) concentrations (0, 2.5, 5, 10, 20, 30, 40, and 50 mg/l.). Samples for assaying enzyme (catechol oxidase and peroxidase) activities were taken three times (on the 8, 12, and 15 day) following chromium application. The results revealed that the level of enzyme activity of most genotypes was increased significantly with increasing chromium concentration. The magnitude of increase in enzymatic activity was also dependent on the genotype and duration of time after chromium treatment. These findings suggest that, the activities of both enzymes might be used as indicator for selecting chromium tolerant sorghum genotypes.
Keywords
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