Limitation of Liver tumor promoting properties of butylated hydroxytoluene in non- transgenic C57BL6 black mouse

Document Type : Original articles

Author

Forensic medicine and toxicology department Faculty of Veterinary medicine, Mansoura University, Egypt

Abstract

It was reported previously that butylated hydroxytoluene (BHT) had tumor promoting properties on c-myc transgenic mouse model of liver and lung cancer. To better understand limitation of BHT inducing liver tumor, the promoter activity of BHT in non-transgenic mouse C57BL6 model of liver cancer of short term toxicity was investigated. 40 male mice C57BL6 were divided into 5 groups; first group received corn oil, 2nd group treated with single dose of 100 mg/kg of diethylnitrisamine, 3rd .4th and 5th group treated with single dose of 100 mg/kg of diethylnitrisamine followed by BHT at doses of 100, 200 and 300 mg/kg respectively twice per week for 32 weeks. Liver to body weight ratio was increased non-significantly in all treated groups in particular at dose of 300 mg/kg of BHT when compared with control group. Furthermore, butylated hydroxytoluene at the highest dose increased significantly (≤0.05) liver transaminase enzymes, alkaline phosphatase, BUN, cholesterol and glucose level when compared with group 2 treated only with DEN or control group. At 32 weeks, Diethylnitrisamine at dose of 100 mg/kg induced liver dysplasia while BHT fail to promote conversion of liver dysplasia to ultimate unicellular or focal liver tumor. Notably BHT enhanced leukocytic infiltration and dysplasia of primary pulmonary cell at dose of 300 mg/kg in histopathology examination. Moreover, on conclusion BHT had no promoting tumor activity in wild mice but butylated hydroxytoluene consider a hazardous chemical for liver and lung tissue at high dose.

Keywords

Scientific Journal for Damietta Faculty of Science
Volume 6, Issue 2
December 2016
Pages 116-121

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