Distribution and molecular identification of Candida albicans isolated from various clinical samples in Egypt.

Document Type : Original articles

Authors

1 Botany and Microbiology , Fcaculty of sciences Damietta Univ.

2 Microbiology and Botany Department, Faculty of Science, Damietta University, Damietta, Egypt

Abstract

Candida albicans is responsible for causing candidiasis in Egyptian women, so a fast and accurate identification is needed for perfect prescription. A total of 15 samples, obtained from vaginal microenvironment of symptomatic women, were cultivated and rejuvenated on a Sabouraud Dextrose Agar (SDA) medium and identified by using the API 20C AUX kit, germ tube test, chromogenic medium and molecular technique. A total of six strains of Candida albicans was isolated from the pregnant and nonpregnant women in Egypt. Eighty percentage of patients are the in the age ranged of 25-40 years and the remaining 20% are in age between 18 -25 and the highest level of infection (60%) in the elder one. Pregnant women are more infected (80%) than non-pregnant (20%). Non-educated ladies are more infected (80%) than educated one (20%). The fungal strains were identified as Candida albicans through growth at 45°C on CHROMagarTM Candida Medium and forming green colonies. Germ tube test and biochemical system using API 20C Aux were used in confirmation. The genomic DNA was extracted from the six samples and rDNA-ITS region was amplified and sequenced. A phylogenetic tree of the fungus was constructed. Both SEM and TEM Electron microscopy analyses were performed for showing Candida albicans structure.

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