Antimicrobial activity of biosynthesized selenium nanoparticles using Pseudomonas aeruginosa isolate

Elshikiby, Laila A.; Baka, Zakaria A.; El-Zahed, Mohamed M.

doi:10.21608/sjdfs.2025.387130.1231

Antimicrobial activity of biosynthesized selenium nanoparticles using Pseudomonas aeruginosa isolate

Document Type : Original articles

Authors

¹ Department of Botany and Microbiology, Faculty of Science, Damietta University, New Damietta city, Egypt

² Department of Botany and Microbiology, Faculty of Science, Damietta University, New Damietta, Egypt.

10.21608/sjdfs.2025.387130.1231

Abstract

The current study used Pseudomonas aeruginosa isolated from clinical specimens to provide a simple and cost-effective approach for biosynthesizing selenium nanoparticles (SeNPs). Vitek 2 was used to confirm the species-level identification of the chosen bacterial isolate after it was recognized traditionally. The biosynthesized SeNPs were verified and described using a variety of tests, including TEM, FT-IR, zeta analyses, and UV-Vis spectroscopy. At 330-370 nm, the adsorption peaks for SeNPs were recorded. The presence of proteins acting as binding and supporting components during synthesis was verified by the FT-IR spectra. The substantial negative surface charge of -17.6 mV contributes to its stability. TEM shows the diameters of SeNPs in the 83-91 nm range. SeNPs had strong antimicrobial activity against Bacillus cereus, Staphylococcus aureus, Proteus mirabilis, Pseudomonas aeruginosa, and Candida albicans in a dose-dependent manner. The MICs of SeNPs against B. cereus, S. aureus, P. aeruginosa, P. mirabilis, and C. albicans were 40, 70, 110, 150, and 150 µg/ml, respectively. This study offers safe, effective, and promising nanoparticles as an alternative for treating some pathogenic microbes in both humans and animals.

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